Protein analysis of : Regenerative salamanders vs. non-regenerative salamanders. Regenerative tadpoles vs. non-regenerative tadpoles Once human regenerative proteins are identified, must develop human specific cell lines to manufacture the proteins.
Develop a mammalian regeneration model using similar proteins identified in salamanders to induce blastema formation in mammals, like rodents.
Use protein analysis to compare rodent wound healing to human wound healing (to identify differences between the two groups that might alter limb regeneration.)
Compare regenerative salamander protein levels vs. non-regenerative frog levels over several hours, days, and weeks. A clear pattern of protein levels may immerge.
Clone cell lines to produce sufficient amounts of protein for treatment.