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1st Slide - Image of dissolvable
suture mold. 2nd Slide - Image of KOT kidney in the earlier stages of development. 3rd Slide - Image of KOT kidney in later stages of development (mold has started to disintegrate). 4th Slide - Image of final KOT kidney, fully formed, with renal artery, renal vein, and ureter attached. (All pictures shown represent a cross section of the KOT kidney). |
Steps to Create KOT:
1.) Design a biodegradable plastic mold for the kidney
- will be made from layers of dissolvable suture(stitches) polymers
- made with high elasticity(so it will stretch) - made with high-tension(so it will sustain pressure) - slow degradation rate(so it will have time for the tissues to develop) - mold will be separated into 5 sections of different sizes for each tissue - 5 layers: -renal capsule -outer cortex -inner cortex -medulla -collecting system that drains into the ureter
2.) Graft the tissues & membranes
- same process as cultured skin grafts
- coat mold in layer of denatured collagen(create grid pattern for cells) - perform surgery to remove healthy samples of tissue - seed healthy tissue, stem cells, & growth factors onto single layer of biodegradable material - each layer grown apart until individual structures form - layers placed in mold in correct order - grow additional nephrons - layers and nephrons will be combined to form a complete kidney - kidney will take three to four months to fully develop
3.) Sustain kidney in a growth medium
- use same medium that is used to sustain skin grafts
- add different growth factors to medium
4.) Transplantation of kidney into patient
- use current kidney transplant procedure
- surgeon will make an incision on either side of the abdomen - kidney will be attached to assorted blood vessels (the iliac artery and vein) - connect the ureter to the bladder - close incision and transport patient to the intensive care unit |
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